Search results for "Specific identification"

PCR ITS-RFLP for Penicillium Species and Other Genera

2016

Among numerous molecular methodologies developed for highly specific identification of filamentous fungi isolates, here we describe restriction digestion analysis of the ITS products as an easy method to identify isolates of filamentous fungi. This technique is a rapid and reliable method appropriate for routine identification of filamentous fungi. This can be used to screen large numbers of isolates from various environments in a short time. The use of different endonucleases allowed generating individual restriction profiles. The individual profiles obtained were combined into composite restriction patterns characteristic of a species. Eleven different genera can be differentiated and amo…

Identification of fish species by reversed-phase high-performance liquid chromatography with photodiode-array detection

1998

A method for the separation of sarcoplasmic fish proteins by RP-HPLC is described. The procedure revealed significant differences useful for reliable identification of fish species. Sixteen of the most common Finnish freshwater fish species were differentiated by species-specific HPLC chromatograms obtained using photodiode-array detection (PAD) at 200-350 nm. The analytical column was a Hi-Pore RP-304 reversed-phase column. The separation was performed by a linear gradient of acetonitrile and water with a small amount of trifluoracetic acid (TFA). Star-symbol plots were constructed from the chromatograms to visualize the data. Clearly different HPLC protein profiles for most fish species w…

Specific Identification and Quantification of the Spoilage Microorganism Brettanomyces in Wine by Flow Cytometry: A Useful Tool for Winemakers

2010

International audience; no abstract

Method for Specific Identification of the Emerging Zoonotic Pathogen Vibrio vulnificus Lineage 3 (Formerly Biotype 3).

2020

ABSTRACT Vibrio vulnificus is a zoonotic pathogen that is spreading worldwide due to global warming. Lineage 3 (L3; formerly biotype 3) includes the strains of the species with the unique ability to cause fish farm-linked outbreaks of septicemia. The L3 strains emerged recently and are particularly virulent and difficult to identify. Here, we describe a newly developed PCR method based on a comparative genomic study useful for both rapid identification and epidemiological studies of this interesting emerging group. The comparative genomic analysis also revealed the presence of a genetic duplication in the L3 strains that could be related to the unique ability of this lineage to produce sept…

Misidentification ofDiphyllobothriumSpecies Related to Global Fish Trade, Europe

2014

To the Editor: Diphyllobothriosis, infection by tapeworms of the genus Diphyllobothrium (Cestoda: Diphyllobothriidea) (1), is a well-known disease of humans. In Europe, infections caused by 3 species of Diphyllobothrium have recently been reported in humans: D. latum is considered to be the principal species infecting persons in Europe (1); 4 cases of D. dendriticum infection and 6 cases of D. nihonkaiense infection have also been reported (2,3). Except for those caused by D. latum, which is autochthonous in northeastern Europe and subalpine lakes, most of the cases in Europe have been imported or caused by consumption of fish imported from areas to which the parasites are endemic (1,3,4). …

SCAR-based real time PCR to identify a biocontrol strain (T1) of Trichoderma atroviride and study its population dynamics in soils.

2006

Strains of Trichoderma spp. are known for their antagonistic properties against plant pathogens, some are already on the market, others are under development. In order to launch a strain on the market its perfect identification at the species and strain levels is needed. The aim of this study is to (i) design a SCAR marker for specific identification of strain T1 of Trichoderma atroviride and (ii) monitor population dynamics of this strain in soil by real time PCR. A primer pair targeting a 141-bp fragment enabled specific detection of this strain without cross detection of autochthonous populations of Trichoderma in several field soils. In two soils, population dynamics assessed by real ti…

Species-specific identification of Dekkera/Brettanomyces yeasts by fluorescently labeled DNA probes targeting the 26S rRNA.

2007

Sequencing of the complete 26S rRNA genes of all Dekkera/Brettanomyces species colonizing different beverages revealed the potential for a specific primer and probe design to support diagnostic PCR approaches and FISH. By analysis of the complete 26S rRNA genes of all five currently known Dekkera/Brettanomyces species (Dekkera bruxellensis, D. anomala, Brettanomyces custersianus, B. nanus and B. naardenensis), several regions with high nucleotide sequence variability yet distinct from the D1/D2 domains were identified. FISH species-specific probes targeting the 26S rRNA gene's most variable regions were designed. Accessibility of probe targets for hybridization was facilitated by the constr…

Notes: FOOD AND PARASITES FROM TWO HOURGLASS DOLPHINS, LAGENORHYNCHUS CRUCIGER (QUOY AND GAIMARD, 1824), FROM PATAGONIAN WATERS

2003

On the Rock Partridge in the painting “Saint Jerome in the study” by Antonello da Messina.

2012

Pictorial work of Antonello da Messina during the Sicilian period (1455-60) is considered less fruitful and less mature than during his stay in Venice (ca 1474-1476). While some art critics consider the painting “Saint Jerome in the study” a work of the first period, others believe that it was produced during the Venice period. The Authors analysed the plumage characters of the Rock Partridge, Alectoris graeca, depicted with symbolic significance in the painting, to try the identification of the population which the subject used as model could belong to. However, they conclude that the identification as a Sicilian as well as an Alpine Rock Partridge is doubtful and in this case the plumage …